The Spanish language derives form Latin, and the Christianity was spread in the Iberian Peninsula at about the second century. Both Latin and Christianity were introduced to Spain by the Roman Empire during the time of colonization. The Christian mentality has since had an influence of great dimension in all the aspects of this country. Especially, throughout the long period of coexisting with the Spanish language, Christianity and the derived religious culture have become intimately integrated with the above mentioned language and have formed a compact union. This fact has reflected in the daily expressions. In this investigation all the biblical quotations will be taken from the Spanish Bible of 1569 version of Casiodoro de Reina(1520-1594), and we will gather and explain the significance of the short phrases in Spanish related directly or indirectly to the Bible.